Journal: International Journal of Molecular Sciences

Article Title: Escherichia coli as a New Platform for the Fast Production of Vault-like Nanoparticles: An Optimized Protocol

doi: 10.3390/ijms232415543

Figure Lengend Snippet: Production of MVP-H6 protein. Total cell pellets obtained from cultures induced either for 3 h at 37 °C ( A , B ) or o/n at 20 °C ( C ) were submitted to SDS-PAGE and further Western blots developed with an anti-His monoclonal antibody ( A , C ), or with an anti-MVP polyclonal antibody ( B ).

Article Snippet: For Western blot analyses, a rabbit polyclonal antibody anti-MVP (ref. ab90009, Abcam, Cambridge, UK) or anti-His mouse monoclonal antibodies (ref. A00186-100, GenScript, Piscataway, NJ, USA, or ref. 631212, Clontech, Mountain View, CA, USA) were used as primary antibodies to detect MVP-H6 protein.

Techniques: SDS Page, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Escherichia coli as a New Platform for the Fast Production of Vault-like Nanoparticles: An Optimized Protocol

doi: 10.3390/ijms232415543

Figure Lengend Snippet: Co-purification of MVP-H6 and GFP-INT proteins. IMAC-based resin was used to purify both MVP-H6 and GFP-INT, as described in Material and Methods. Images correspond to SDS-PAGE and Western blot developed with an anti-His monoclonal antibody ( A ) or a polyclonal antibody anti-GFP ( B ). Legends: initial sample (I), flowthrough (F), wash (W) and eluted (E).

Article Snippet: For Western blot analyses, a rabbit polyclonal antibody anti-MVP (ref. ab90009, Abcam, Cambridge, UK) or anti-His mouse monoclonal antibodies (ref. A00186-100, GenScript, Piscataway, NJ, USA, or ref. 631212, Clontech, Mountain View, CA, USA) were used as primary antibodies to detect MVP-H6 protein.

Techniques: Copurification, SDS Page, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Escherichia coli as a New Platform for the Fast Production of Vault-like Nanoparticles: An Optimized Protocol

doi: 10.3390/ijms232415543

Figure Lengend Snippet: Trypsin digestion of GFP-His and GFP-INT. Samples at the indicated time points (in minutes) were taken and analyzed by SDS-PAGE and Western blot developed with a polyclonal antibody anti-GFP ( A ) detecting both GFP-H6 and GFP-INT. Kinetics of trypsin degradation according to densitometric analysis of Western blots is shown in ( B ). Results shown represent the mean and standard error of the mean (SEM) of two independent samples analyzed by duplicate.

Article Snippet: For Western blot analyses, a rabbit polyclonal antibody anti-MVP (ref. ab90009, Abcam, Cambridge, UK) or anti-His mouse monoclonal antibodies (ref. A00186-100, GenScript, Piscataway, NJ, USA, or ref. 631212, Clontech, Mountain View, CA, USA) were used as primary antibodies to detect MVP-H6 protein.

Techniques: SDS Page, Western Blot

Journal: Cell

Article Title: The mammalian ribo-interactome reveals ribosome functional diversity and heterogeneity

doi: 10.1016/j.cell.2017.05.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-MVP , Proteintech Group , Cat#16478-1-AP; RRID: AB_2147597.

Techniques: Recombinant, Membrane, Protein Extraction, Reporter Assay, Software